hfls medium  (Cell Applications Inc)

Journal: Journal of Clinical Medicine

Article Title: Adiponectin Is a Component of the Inflammatory Cascade in Rheumatoid Arthritis

doi: 10.3390/jcm11102740

Figure Lengend Snippet: Effect of stimulation of human fibroblast-like synoviocytes (HFLSs) with LPS, TNF-α, and LPS + TNF-α on adiponectin mRNA expression. Means from 3 experiments ± 95% confidence intervals are presented. * p < 0.05 (Tukey post hoc test in comparison to non-stimulated control). TNF: Tumor necrosis factor. LPS: Lipopolysaccharide.

Article Snippet: Afterwards, the medium was replaced by HFLS Basal Medium (Cell Applications, Inc., San Diego, CA, USA) containing low levels of bovine serum albumin (BSA; 0.5%) (Sigma) to render the cells quiescent for 24 h. The cells were washed with PBS (EURx) the next day, and stimulating factors were added.

Techniques: Expressing, Comparison, Control

Journal: Journal of Clinical Medicine

Article Title: Adiponectin Is a Component of the Inflammatory Cascade in Rheumatoid Arthritis

doi: 10.3390/jcm11102740

Figure Lengend Snippet: The effect of adiponectin (ADIPO) stimulation of human fibroblast-like synoviocytes (HFLSs) on IL-6 mRNA expression. Red arrow indicates negative control (without adiponectin and without LPS). Black arrow indicates control with LPS alone (without adiponectin). Means from 3 experiments ± 95% confidence intervals are presented. * p < 0.05 (Tukey post hoc test in comparison to cells not stimulated with adiponectin).

Article Snippet: Afterwards, the medium was replaced by HFLS Basal Medium (Cell Applications, Inc., San Diego, CA, USA) containing low levels of bovine serum albumin (BSA; 0.5%) (Sigma) to render the cells quiescent for 24 h. The cells were washed with PBS (EURx) the next day, and stimulating factors were added.

Techniques: Expressing, Negative Control, Control, Comparison